Sorting of spermatogonial stem cells in mice / 中华男科学杂志
National Journal of Andrology
;
(12): 24-26, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-289054
ABSTRACT
<p><b>OBJECTIVE</b>To search for an effective method for enriching spermatogonial stem cells in mice.</p><p><b>METHODS</b>Bilateral artificial cryptorchidism was performed on 20 six-week old male Kunming mice. Three months after the operation, the testes were removed and single cell suspension prepared by two-step enzyme digestion. FITC-conjugated anti-alpha6-integrin antibody and PE-conjugated anti-c-kit antibody were added for adequate time on ice. Then the cells with low side scatter light-scattering properties were sorted and positively stained for alpha6-integrin and negative c-kit expression. And the viability of the isolated cells was assessed by trypan blue exclusion.</p><p><b>RESULTS</b>The sorted spermatogonial stem cells constitute 2.8% of the testis cells and over 95% of them were viable.</p><p><b>CONCLUSION</b>FACS can be used to isolate quantities of viable spermatogonial stem cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermatogonia
/
Stem Cells
/
Random Allocation
/
Cell Separation
/
Cryptorchidism
/
Cell Biology
/
Disease Models, Animal
/
Flow Cytometry
/
Fluorescence
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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