Construction and expression of ATP50 fluorescent protein in TM3 mouse Leydig cells / 中华男科学杂志
National Journal of Andrology
;
(12): 992-996, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-289093
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and localization of ATP50 by construction of ATP50-pEYFP-N1 in primary cultured mouse Leydig cells.</p><p><b>METHODS</b>Primary cultured mouse Leydig cells were confirmed by 3B-HSD staining. ATP50 was cloned into pEYFP-N1 between Bam HI and Eco RI sites. Cell-transfection and living-cell fluorescence imaging microscopy were employed to investigate the sub-cellular localization of YFP-ATP50 in TM3 mouse Leydig cells.</p><p><b>RESULTS</b>ATP50 green fluorescent protein was well co-localized with red fluorescence mitochondrion marker-Mitotracker in TM3 mouse Leydig cells.</p><p><b>CONCLUSION</b>ATP50 was expressed in primary cultured mouse Leydig cells. The fluorescent expression vector of ATP50 was constructed successfully and YFP-ATP50 was located in mitochondria in TM3 mouse Leydig cells, which provided a useful clue for further research on the steroidogenesis dysfunction in aging males.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Transfection
/
Carrier Proteins
/
Cells, Cultured
/
Cloning, Molecular
/
Adenosine Triphosphatases
/
Green Fluorescent Proteins
/
Genetic Vectors
/
Genetics
/
Leydig Cells
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2006
Type:
Article
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