Role of bFGF and TGF-beta1 in primary cultured prostatic stromal cells / 中华男科学杂志
National Journal of Andrology
;
(12): 917-922, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-289109
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of the basic fibroblast growth factor (bFGF) and transforming growth factor beta1 (TGF-beta1) in benign prostatic hyperplasia (BPH).</p><p><b>METHODS</b>The human stromal cells of BPH were isolated and cultured. The proliferation of the stromal cells cultured in serum-free medium was detected by MTT method, the phenotype changes of smooth muscle cells detected by immunohistochemical method, and the effect of different concentrations of bFGF and TGF-beta1 on the cultured stromal cells of BPH observed.</p><p><b>RESULTS</b>bFGF stimulated the cultured BPH stromal cell proliferation (P < 0.05, P < 0.01) and decreased the expression of smooth muscle cell (SMC) phenotype in higher concentration (10 microg/L). TGF-beta1 (> 1 microg/L) inhibited stromal cell proliferation and increased the expression of SMC phenotype (P < 0.05, P < 0.01). 5 microg/ml bFGF and TGF-beta1 (0.001 microg/L, 0.01 microg/L) promoted stromal cell proliferation (P < 0.01), while 5 microg/L bFGF and TGF-beta1 (0.1 microg/L, 1 microg/L, 10 microg/L) inhibited it, slightly in 0.1 microg/L (P > 0.05) and significantly in 1 microg/L and 10 microg/L (P < 0.01), and increased the expression of SMC phenotype in higher concentration (> 1 microg/L, P < 0.01).</p><p><b>CONCLUSION</b>bFGF stimulates the proliferation of the prostatic stromal cells of BPH in a time- and dose-dependent fashion and decreases the expression of SMC phenotype, TGF-beta1 inhibits the growth of stromal cells and induces the differentiation of stromal cells to SMC, both playing an important role in the mechanism of BPH.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Prostatic Hyperplasia
/
Cells, Cultured
/
Fibroblast Growth Factor 2
/
Stromal Cells
/
Cell Biology
/
Cell Proliferation
/
Dose-Response Relationship, Drug
/
Transforming Growth Factor beta1
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS