Role of MAPK signal transduction pathway phosphorylation induced by granulocyte-macrophage-colony stimulating factor in the proliferation of hormone-independent prostate cancer / 中华男科学杂志
National Journal of Andrology
;
(12): 872-875, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-289121
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the phosphorylation intensity of MAPK pathway molecular Erk1/2 and the proliferation of prostate cancer cell line PC-3M.</p><p><b>METHODS</b>Flow cytometry and RT-PCR were employed to study the ratio of different cell cycles and phases, respectively, before and after GM-CSF stimulation. Erk1/2 phosphorylation intensity was examined by Western blot simultaneously.</p><p><b>RESULTS</b>The rate of PC-3M cells at S and G2/M stages and the expression intensity of Ki-67 increased after GM-CSF incubation in a dose-dependent manner. The phosphorylation intensity of Erk1/2 increased remarkably after stimulation with GM-CSF.</p><p><b>CONCLUSION</b>The intensification of Erk1/2 phosphorylation is one important molecular mechanism of the proliferation of hormone-independent prostate cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Phosphorylation
/
Physiology
/
Prostatic Neoplasms
/
Signal Transduction
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Ki-67 Antigen
/
Mitogen-Activated Protein Kinase 1
/
Mitogen-Activated Protein Kinase Kinases
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2006
Type:
Article
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