Influence of peritoneal dialysate on nitric oxide production and viability of macrophage / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 23-25, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-289331
ABSTRACT
OBJECTIVE:
To investigate the influence of commercial peritoneal dialysate (CDS) on function of macrophage.METHODS:
Cultured peritoneal macrophages were divided into two experimental groups and their controls.(1) Macrophages were cultured in conditioned culture medium containing 50%CDS (0.139 mol/L glucose) for 24 h. (2)Macrophage were exposed to CDS containing 0.139mol/L glucose for 10, 30 and 60 min respectively, and then cultured in CDS-free medium for 24 h. The nitric oxide (NO) production and MTT in two groups were measured. In each control group, CDS was replaced by same amount of culture medium.RESULTS:
NO production and MTT reduction ability (related to cell viability) of experimental groups were remarkably lower than those of controls and the NO production and MTT reduction in 60min CDS-exposed group were lower than that of 10 min and 30 min CDS-exposed group.CONCLUSION:
Dialysate may have detrimental effects on viability and other function of macrophage and these effects may related to time length of CDS exposure.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article
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