Nitric oxide mediates the inhibitory effects of interleukin-2 on the isolated ventricular papillary muscle and cultured neonatal rat cardiomyocyte / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 19-22, 2002.
Article
in Zh
| WPRIM
| ID: wpr-289332
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WPRO
ABSTRACT
OBJECTIVE: To explore the effects and mechanism of interleukin-2 on the isolated rat ventricular papillary muscle and cultured cardiomyocyte. METHODS: The isolated rat papillary muscle and cultured neonatal rat cardiomyocyte were used to determine the effects on contractile properties and spontaneous beating. RESULTS: IL-2 inhibited the contractile force of isolated rat papillary muscle and reduced the spontaneous beating rate of cultured neonatal rat cardiomyocytes in a dose-dependent manner. IL-2 at high concentration reduced the systolic duration and prolonged the diastolic duration. Pretreatment with L-NAME, an inhibitor of nitric oxide synthase,abolished the effects of IL-2. Heat inactivated IL-2 had no effect on papillary muscles and cardiomyocytes.CONCLUSION: Inhibitory effects of IL-2 on the contraction of isolated papillary muscle and spontaneous beating rate of cultured neonatal cardiomyocyte are mediated by nitric oxide.
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Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article