Construction of bi-cistronic co-expression plasmid of mIL-12 and the expression in vitro or in vivo / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 15-18, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-289333
ABSTRACT
OBJECTIVE:
To construct a bi-cistronic co-expression plasmid for mouse interleukin-12 and to observe its expression in vitro or in vivo.METHODS:
The full-length cDNA encoding p35 and p40 was cloned into eukaryotic cells expression vector pcDNA 3.1 respectively. Subsequently,the p35 expression unit was inserted into pcDNA 3.1/p40 to produce the bi-cistronic co-expression plasmid in which the p35 and p40 genes were controlled by their own CMV.The plasmid was expressed in vitro and in vivo.RESULTS:
The mIL-12 in the supernatant was detected by ELISA after the pCmIL-12 was transfected into COS-7 cells. The activity of NK cells could be augmented by the supernatant in vitro and also by by intradermal delivery of pCmIL-12 in vivo.CONCLUSION:
The plasmid constructed by us can express biologically active mIL-12 in vitro and in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article
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