Novel quantitative method for evaluating oxygen/glucose deprivation-induced injury of hippocampal slices / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 81-85, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-289350
ABSTRACT
OBJECTIVE:
To establish a simple, sensitive in vitro method to evaluate oxygen/glucose deprivation (OGD)-induced injury of brain hippocampal slices in rats.METHODS:
Rat hippocampal slices were incubated in 2% 2, 3, 5-triphenyltetrazolium chloride (TTC) solution after oxygen/glucose deprivation. They were then soaked in a measured volume of ethanol and dimethylsulfoxide (5050) to extract the TTC formazan Product which was then measured by spectrophotometry. OGD induced LDH release was simultaneously measured.RESULTS:
Progressive prolongation of OGD induced hippocampal injury resulted in decreased formazan coloration as determined by spectrophotometry. There was a parallel increase in LDH release, thus a negative correlation in these two products was noted. (r=-0.933,P <0.01). The injury was attenuated in the brain slices pre-treated with nimodipine, dexamethasone, and ketamine, but not ONO-1078.CONCLUSION:
Solvent extraction and spectrophotometric quantification of formazan represents an objective measurement of OGD-induced injury of rat hippocampal slices.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article
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