Preliminary functional analysis of intron in chalcone synthase gene from Scutellaria baicalensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 361-365, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-289366
ABSTRACT
<p><b>OBJECTIVE</b>To study the function of the chalcone synthese gene introns in Scutellaria baicalensis, and clarify preliminarily their role in abiotic stress.</p><p><b>METHOD</b>The CHS introns with specific primers were cloned and bioinformatic method was applied to predict the cis-elements in the intron of CHS. The introns were subcloned into binary vector, pCAMBIA-1301 before being transferred to tobacco. Then the activity of GUS of the transgenic tobacco seeds was analyzed.</p><p><b>RESULT</b>Seven cis-elements were found in the introns. Under the dark and high temperature GUS expression rose at the first (3 h), but then declined (9 h). ABA and MeJA regulated insignificantly the GUS activity in normal temperature; treatment of 10% PEG induced GUS expression.</p><p><b>CONCLUSION</b>CHS introns could be play a role in the regulation of S. baicalensis phenylpropanoid biosynthetic pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Polyethylene Glycols
/
Nicotiana
/
Acyltransferases
/
Molecular Sequence Data
/
Base Sequence
/
Introns
/
Regulatory Sequences, Nucleic Acid
/
Sequence Alignment
/
Plants, Genetically Modified
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS