Effect of jinqiaomai on expression of TLR2/4, MyD88 mRNA and IkappaB-alpha in lung tissue of rats with Klebsiella pneumonia / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 200-204, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-289399
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of protective effect of Jinqiaomai (JQM) on lung injury induced by Klebsiella pneumonia in rats.</p><p><b>METHOD</b>The model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, JQM (6, 3, 1.5 g x kg(-1)) three groups, levofloxacin (25 mg x kg(-1)) group, JQM (3 g x kg(-1)) + levofloxacin (25 mg x kg(-1)) group. The contents of IL-1beta, ICAM-1 and INF-gamma in the lung tissue homogenate were measured by radio-immunoassay and Elisa. TLR2/4 mRNA and MyD88 mRNA expression were detected by RT-PCR. IkappaB-alpha expression was detected by Western Blot.</p><p><b>RESULT</b>The rats of model group had obvious lung injury, but those of JQM, JQM + levofloxacin and levofloxacin groups had less injury. The contents of IL-1beta, ICAM-1 and INF-gamma in lung tissue homogenate and the expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha in lung of model group were significantly higher than those in the control group (P < 0.05 or P < 0.01), while IL-1beta, ICAM-1 and INF-gamma of JQM groups were significantly lower than those of model group (P < 0.05 or P < 0.01). The expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha of JQM (6.3 g x kg(-1)) groups were significantly lower than those of model group(P <0. 05 or P <0. 01).</p><p><b>CONCLUSION</b>The lung injury induced by Klebsiella pneumonia is related to TLR2/4, MyD88 mRNA and IkappaB-alpha. To decrease the excessive expression of TLR2/4, MyD88 mRNA and IkappaB-alpha might be the main mechanism of protective effect of Jinqiaomai on lung injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Klebsiella Infections
/
Drugs, Chinese Herbal
/
Random Allocation
/
Gene Expression
/
Rats, Sprague-Dawley
/
I-kappa B Proteins
/
Disease Models, Animal
/
Drug Therapy
/
Toll-Like Receptor 2
Type of study:
Controlled clinical trial
/
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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