Oxysophoridine suppresses the growth of hepatocellular carcinoma in mice: in vivo and cDNA microarray studies / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 209-213, 2012.
Article
in English
| WPRIM
| ID: wpr-289654
ABSTRACT
<p><b>OBJECTIVE</b>To observe the in vivo effects of oxysophoridine on hepatocellular carcinoma in mice and to study the related mechanisms.</p><p><b>METHODS</b>C57BL mice were inoculated with mouse hepatoma H22 cells subcutaneously, then divided into 5 groups (14 per group), and treated with oxysophoridine (50, 100, or 150 mg/kg) or cisplatin (4 mg/kg) for 10 days. Inhibitory rate of tumor, body weight gain, and influence indices on internal organs (liver, spleen and thymus) were evaluated. The differentially expressed genes between the oxysophoridine-treated group, and the control group were analyzed using cDNA microarray and quantitative real-time PCR (qRT-PCR) experiments.</p><p><b>RESULTS</b>Compared with the tumor weight of the control group (2.75±0.66 g), oxysophoridine significantly suppressed hepatocellular carcinoma growth in mice (P <0.01), with 0.82±0.36 g, 0.57±0.22 g, and 1.22±0.67 g for the tumor weight in the low, moderate, and high dose treatment group, respectively. The moderate dose led to the highest inhibitory rate, 79.3%. Observation of body weight gain and influence on three organs showed that compared with cisplatin, oxysophoridine produced fewer side effects in vivo. cDNA microarray and qRT-PCR showed that the most significant differentially expressed genes in the tumor samples of oxysophoridine-treated mice were mostly involved in regulating apoptosis, with the Tnfrsf11b (osteoprotegerin) gene being the most significantly affected.</p><p><b>CONCLUSION</b>Oxysophoridine was a promising compound for developing drugs against hepatocellular carcinoma, and its anti-hepatoma effect was probably related to osteoprotegerin activation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Specificity
/
Pathology
/
Pharmacology
/
Drug Screening Assays, Antitumor
/
Weight Gain
/
Gene Expression Regulation, Neoplastic
/
Carcinoma, Hepatocellular
/
Oligonucleotide Array Sequence Analysis
/
Cell Line, Tumor
/
Tumor Burden
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2012
Type:
Article
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