Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 283-287, 2012.
Article
in English
| WPRIM
| ID: wpr-289669
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of puerarin on interleukin (IL)-8 mRNA expression and the protein release in the co-culture of human bronchial epithelial (BEAS-2B) cells and human neutrophils.</p><p><b>METHODS</b>BEAS-2B cells and neutrophills were cultured separately and co-cultured with puerarin (50, 100, and 200 μg/mL) for a predetermined time. Cytokines in culture supernatant were evaluated by protein array and IL-8 quantified by enzyme-linked immunosorbent assay (ELISA). IL-8 mRNA expression was evaluated by real-time quantitative polymerase chain reaction (real-time qPCR).</p><p><b>RESULTS</b>The co-culture of BEAS-2B cells and neutrophils exhibited synergistic effects on IL-8 mRNA expression in BEAS-2B cells, but not in neutrophils after 12 h incubation (P<0.01), as compared with that in BEAS-2B cells or neutrophils alone. IL-8 protein release in the culture supernatant was obviously elevated when BEAS-2B cells were co-cultured with human neutrophils as compared with that in the supernatant of BEAS-2B cells or neutrophils alone after incubated for 2, 6, 12, and 18 h (P<0.01). Treatment with puerarin could significantly down-regulate the expression of IL-8 mRNA in BEAS-2B cells and IL-8 release in the supernatant of the co-culture of BEAS-2B cells and neutrophils (P<0.01).</p><p><b>CONCLUSION</b>Puerarin could exhibit anti-inflammatory activity by suppressing IL-8 production from the co-culture of human bronchial epithelial cells and neutrophils.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bronchi
/
RNA, Messenger
/
Cell Communication
/
Cell Line
/
Gene Expression Regulation
/
Interleukin-8
/
Coculture Techniques
/
Bodily Secretions
/
Cell Biology
Limits:
Adult
/
Humans
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS