Xiongshao capsule promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 840-846, 2011.
Article
in En
| WPRIM
| ID: wpr-289736
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the angiogenic effect of the Xiongshao capsule (XSC) in human umbilical vein endothelial cells (HUVEC), and to investigate the possible molecular mechanisms mediating its biological effect.</p><p><b>METHODS</b>Serum pharmacology was applied in this study, in which different doses of XSC were administrated to rats orally and then XSC-containing serum (XSC-S) was collected for the following in vitro experiments. The viability of HUVEC was determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Cell density was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with propidium iodide staining was performed to determine cell cycle phase. Cell migration was determined by wound-healing method. Capillary tube formation by HUVEC was examined using ECMatrix gel-based assay. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) expression levels were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbant assay (ELISA) analyses.</p><p><b>RESULTS</b>XSC-S dose-dependently stimulated proliferation of HUVEC by promoting the cell cycle G1 to S progression. In addition, XSC-S treatment dramatically increased the migration and capillary tube formation of HUVEC in a dose-dependent manner. Moreover, XSC-S enhanced the expression of VEGF and bFGF at both mRNA and protein levels.</p><p><b>CONCLUSION</b>XSC can promote several features of angiogenesis in endothelial cells through up-regulating the expression of bFGF and VEGF, suggesting that XSC may be a potential novel therapeutic agent for the treatment of ischemic heart diseases.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Proteoglycans
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Capsules
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Drugs, Chinese Herbal
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Up-Regulation
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Cell Movement
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Cell Survival
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Fibroblast Growth Factor 2
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S Phase
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Collagen
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Chinese journal of integrative medicine
Year:
2011
Type:
Article