Bioinformatic and Expression Analysis of Ventricular Septal Defect-associated Long Non-coding RNA TUC40- / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the potential role of TUC40- in human and mouse embryonic heart development.</p><p><b>METHODS</b>Bioinformatics databases including NCBI,UCSC,and Uniprot and software including Clustal,DNAMAN,and MEGA 6 were used to collect information of TUC40- and uc.40-. The expression profile at key time points of heart development was investigated by strand-specific quantitative real time polymerase chain reaction.</p><p><b>RESULTS</b>Uc.40- was conservative in sequence, genomic location, and transcription factor binding sites across human and mouse. Pbx1/TUC40- showed negative trend during embryonic mouse heart maturation.</p><p><b>CONCLUSIONS</b>Various levels of conservation of uc.40- suggests similar functions of TUC40- in these two species. TUC40- may play its roles in human and mouse embryonic heart development by regulating Pbx1.</p>