Mechanisms of foam cell formation in smooth muscle cell derived from bone marrow stem cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1250-1252, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-289948
ABSTRACT
<p><b>OBJECTIVE</b>To establish a model of smooth muscle cells differentiated from bone mesenchymal stem cells (BMSC-SMCs) in vitro and explore the relationship between scavenger receptors A (SR-A) and caveolin-1.</p><p><b>METHODS</b>BMSCs were isolated from the femoral bone of SD rats by adherent culture. After treatment of the BMSC-SMCs with 80 mg/L ox-LDL for 72 h, Western blotting was performed to detect the expression of scavenger receptor SR-A, cell cholesterol transport protein ATP-binding cassette transporter Al (ABCA1) and caveolin-1.</p><p><b>RESULTS</b>BMCS-SMCs became foam cells after treatment with ox-LDL. BMSC-SMC gave rise to more foam cell formation than VSMCs did. Western blotting showed that treatment with 80 mg/L ox-LDL for 72 h resulted in significantly increased expression of SR-A and significantly decreased expressions of ABCA1 and caveolin-1.</p><p><b>CONCLUSIONS</b>Treatment of BMCS-SMCs with ox-LDL results in cholesterol ester accumulation in the cells to result in foam cells, the mechanism of which involves up-regulation of scavenger receptor SR-A expression and down-regulation of the reverse cholesterol transport protein ABCA1 and caveolin-1 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
ATP-Binding Cassette Transporters
/
Cell Biology
/
Caveolin 1
/
Scavenger Receptors, Class A
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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