Hath1 gene transfer inhibits the proliferation of colonic cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1005-1008, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-289980
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Hath1 gene transfer on the proliferation of colonic cancer cells in vitro.</p><p><b>METHODS</b>The recombinant plasmid pcDNA3.1(+)-Hath1 was transfected into HT29 colonic cancer cells, and 3 positive cell clones were randomly selected to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time RT-PCR and Western blotting. The proliferation of the transfected HT29 cells was observed by means of colony formation assay and xenograft growth in nude mice.</p><p><b>RESULTS</b>Hath1 significantly down-regulated of cyclin D1 and up-regulate of p27 expressions and inhibited the proliferation of HT29 cells.</p><p><b>CONCLUSION</b>Hath1 gene may be an anti-oncogene in colon carcinogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Colonic Neoplasms
/
Gene Transfer Techniques
/
Proliferating Cell Nuclear Antigen
/
Cyclin D1
/
Cell Line, Tumor
/
Cell Proliferation
/
Basic Helix-Loop-Helix Transcription Factors
/
Mucin-2
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS