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Effect of imatinib at different concentrations on rat C6 glioma cell apoptosis and cell cycle / 南方医科大学学报
Journal of Southern Medical University ; (12): 1089-1091, 2010.
Article in Chinese | WPRIM | ID: wpr-289985
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of imatinib on rat C6 glioma cell apoptosis and cell cycle.</p><p><b>METHODS</b>MTT assay was used to determine the OD value of C6 glioma cells following treatment with imatinib at different concentrations (0.156, 10 and 15 micromo/L) for 24, 48 and 72 h. The cell apoptosis was assayed by Hochest/PI staining and the cell cycle changes were analyzed by flow cytometry.</p><p><b>RESULTS</b>Imatinib treatment resulted in increased number of apoptotic cells in a time- and dose-dependent manner. A 72-h treatment of the cells with imatinib at 10 and 15 micromo/L caused increased cell percentage in G(0)/G(1) phase to (68.53-/+0.83)% and (70.41-/+0.62)%, (P<0.01), decreased the percentage of G(2) phase cells to (14.48-/+0.12)% and (13.84-/+2.86)% (P<0.01), and decreased the percentage of S phase cells to (16.98-/+0.72)% and (15.78-/+2.28)%, respectively (P<0.01).</p><p><b>CONCLUSION</b>Imatinib can induce apoptosis and affect the distribution of the cell cycle of C6 cells in vitro.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Piperazines / Pyrimidines / Benzamides / Cell Cycle / Apoptosis / Cell Line, Tumor / Dose-Response Relationship, Drug / Imatinib Mesylate Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Piperazines / Pyrimidines / Benzamides / Cell Cycle / Apoptosis / Cell Line, Tumor / Dose-Response Relationship, Drug / Imatinib Mesylate Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2010 Type: Article