Preparation of RNA probe for cd99l2 gene of zebrafish labeled with digoxingenin-UTP / 南方医科大学学报
Journal of Southern Medical University
;
(12): 969-972, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-290017
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression pattern of cd99l2 gene during zebrafish development, the RNA probes for whole-mount in situ hybridization were prepared in this study.</p><p><b>METHODS</b>The cd99l2 fragment obtained by RT-PCR was cloned into pGM-T Easy, then the plasmids were linearized with the restriction enzymes SacII or SalI. Using Sp6 or T(7) RNA polymerase, the digoxingenin-labeled antisense and sense probes were synthesized and confirmed by whole-mount in situ hybridization.</p><p><b>RESULTS</b>The plasmid cd99l2/pGM-T was constructed. cd99l2 gene expression pattern during embryogenesis of zebrafish was examined using the antisense probe, and intense expression was detected in the central nervous system during zebrafish development.</p><p><b>CONCLUSION</b>The antisense probe can be used for study of the spatial and temporal distribution of cd99l2 during zebrafish development using the sense probe as control.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Uridine Triphosphate
/
Zebrafish
/
Oligonucleotide Probes
/
RNA Probes
/
Central Nervous System
/
Chemistry
/
Embryology
/
Cloning, Molecular
/
Gene Expression Regulation, Developmental
/
Zebrafish Proteins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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