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Expression of p53, p21(CIP1/WAF1) and eIF4E in the adjacent tissues of oral squamous cell carcinoma: establishing the molecular boundary and a cancer progression model / 国际口腔科学杂志·英文版
Article in En | WPRIM | ID: wpr-290161
Responsible library: WPRO
ABSTRACT
The present study evaluated the expression of key molecules and the status of DNA in both oral squamous cell carcinoma (OSCC) and adjacent tissues to establish a molecular surgical boundary and provide a cancer progression model. Biopsy samples from 50 OSCC patients were divided into T (cancer), P1 (0-0.5 cm), P2 (0.5-1 cm), P3 (1-1.5 cm) and P4 (1.5-2 cm) groups based on the distances from the visible boundary of the primary focus. Twenty samples of normal mucosa were used as controls. We used immunohistochemical staining and flow cytometry to evaluate p53, p21(CIP1/WAF1), eIF4E and Ki-67 expression and to determine DNA status, respectively. Sub-mucosal invasion was present in the P1 and P2 groups as determined by haematoxylin and eosin staining. Mutant p53 expression decreased gradually from cancerous to normal mucosae, whereas p21(CIP1/WAF1) expression displayed an opposite trend. eIF4E expression decreased from cancerous to normal mucosae. Ki-67 expression, the heteroploidy ratio, S-phase fraction and proliferative index decreased gradually with the distance from the tumour centre. Based on these results, we suggest that the resection boundary in OSCC surgery should be beyond 2 cm from the tumour. Additionally, the adjacent tissues of the primary focus could be used as a model for assessing cancer progression.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Pathology / Mouth Neoplasms / Carcinoma, Squamous Cell / Tumor Suppressor Protein p53 / Disease Progression / Eukaryotic Initiation Factor-4E / Cyclin-Dependent Kinase Inhibitor p21 / Metabolism Type of study: Prognostic_studies Limits: Adult / Aged / Female / Humans / Male Language: En Journal: International Journal of Oral Science Year: 2015 Type: Article
Full text: 1 Index: WPRIM Main subject: Pathology / Mouth Neoplasms / Carcinoma, Squamous Cell / Tumor Suppressor Protein p53 / Disease Progression / Eukaryotic Initiation Factor-4E / Cyclin-Dependent Kinase Inhibitor p21 / Metabolism Type of study: Prognostic_studies Limits: Adult / Aged / Female / Humans / Male Language: En Journal: International Journal of Oral Science Year: 2015 Type: Article