The inhibitory effects of an antisense u-PAR vector on invasion of highly invasive human prostate carcinoma PC-3M cell subclones / 华中科技大学学报(医学)(英德文版)
J. huazhong univ. sci. tech. med. sci
; (6): 101-104, 2003.
Article
in En
| WPRIM
| ID: wpr-290503
Responsible library:
WPRO
ABSTRACT
To observe the inhibitory effects of an antisense u-PAR vector on invasion of highly invasive PC-3M cell subclones, the effects of the antisense u-PAR on activity of MMP-9 in those highly invasive cell subclones were detected by a quantitative RT-PCR and zymography. The monolayer invasion assay and colony formation assay in soft agar were used. And tumorigenesis rate and invasions by the cell subclones with or without the antisense u-PAR were observed in nude mice. It was found that in vitro growth of highly invasive PC-3M cell subclones transfected with the antisense u-PAR was declined, and the ability of anchorage-independent growth of those cell subclones was found decreased sharply, with the inhibiting rate becoming 79% and 60%, respectively. Although the antisense u-PAR didn't change MMP-9 gene transcription, they could inhibit the activation of MMP-9 of highly invasive PC-3M cell subclones. Moreover, the tumorigenesis rate of the cell subclones with the antisense u-PAR decreased and the growth of a neoplasm also slowed down. The t tests showed the difference between experimental and control groups was statistically significant (P < 0.01). The antisense u-PAR vector could not only inhibit the invasion ability of highly invasive PC-3M cell subclones in vitro but also restrain the growth of those cell subclones in vivo.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Prostatic Neoplasms
/
Tumor Cells, Cultured
/
Transfection
/
Cell Division
/
Antisense Elements (Genetics)
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RNA, Antisense
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Cloning, Molecular
/
Receptors, Cell Surface
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
J. huazhong univ. sci. tech. med. sci
Year:
2003
Type:
Article