Synthesis of cholecystokinin peptide CCK-4 exclusively by enzymatic methods / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 285-287, 2002.
Article
in English
| WPRIM
| ID: wpr-290533
ABSTRACT
The synthesis of CCK-4 (H-Trp-Met-Asp-Phe-NH2) by using enzymes exclusively was described. As protection group for the amino group we used the Phenylacetyl group (Phac) which had been cleaved at the end of the synthesis with Penicillin G Amidase (PGA) without affecting the peptide bonds. Thus, beginning with Phac-Trp-OH we had successfully synthesized the target peptide with following 4 enzymes, alpha-Chymotrypsin, Papain, Thermolysin and PGA in four reaction steps. All reactions were carried out in aqueous buffer in reasonable yields (> 65%). FAB-MS or FD-MS verified the correct molecular mass of all peptides.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Tetragastrin
/
Chymotrypsin
/
Papain
/
Thermolysin
/
Enzymes, Immobilized
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2002
Type:
Article
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