Expression features of glypican-3 and its diagnostic and differential values in hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 260-265, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-290619
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression features of glypican-3 (GPC-3) and its diagnostic and differential values in hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Rat hepatoma models were made and the dynamic expression features of GPC-3 protein and its gene were investigated by Western blotting and RT-PCR respectively. Liver specimens from 36 HCC patients were collected by self-control method and the expression and clinicopathological features of GPC-3 were analyzed by immunohistochemistry. Serum GPC-3 levels were quantitatively detected by ELISA and its efficiency for HCC diagnosis was evaluated in patients with liver diseases.</p><p><b>RESULTS</b>The incidence of GPC-3 was 0% in control, 83.3% in degeneration, 100% in precancerosis and 100% in canceration during dynamic formation of rat hepatoma, respectively. The positive GPC-3 was brown granule- like staining localized in membrane and cytoplasm in human HCC.</p><p><b>CONCLUSIONS</b>The GPC-3 positive rates were 80.6% in HCC, 41.7% in surrounding tissues and none in distal tissues (P < 0.01), respectively. No positive relationship presented between GPC-3 and differentiation grade or the number of tumor except of tumor size (Z = 2.941, P < 0.01). The incidence of serum GPC-3 was 52.8% in HCC patients except of one patient with cirrhosis. No significant differences were found between GPC-3 and sex, age, AFP, tumor number, Child classification or extrahepatic metastasis except of tumor size (χ² = 6.318, P < 0.05) and HBV infection (χ² = 23.362, P < 0.01). Combined detection of GPC-3 and AFP could rise up diagnosis of HCC. GPC-3 expression closely associated with HCC and might be useful for early diagnosis of HCC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Rats, Sprague-Dawley
/
Carcinoma, Hepatocellular
/
Diagnosis
/
Diagnosis, Differential
/
Glypicans
/
Liver
/
Liver Neoplasms
/
Metabolism
Type of study:
Diagnostic study
/
Screening study
Limits:
Aged80
/
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2011
Type:
Article
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