Effects of Survivin-T34A mutant on breast cancer cell in vitro and in vivo / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 648-651, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-290699
ABSTRACT
To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the experiment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Survivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9-null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow cytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47.1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Breast Neoplasms
/
Transfection
/
Injections, Intralesional
/
Apoptosis
/
Drug Therapy
/
Inhibitor of Apoptosis Proteins
/
Genetics
/
Mice, Nude
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS