Screening and identification of genes associated with multi-drug resistance in colonic cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 388-391, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-290777
ABSTRACT
<p><b>OBJECTIVE</b>To identify novel multi-drug resistance-related genes, and to explore the mechanisms of multi-drug resistance.</p><p><b>METHODS</b>Multi-drug resistant cell line Lovo/5-FU was established by incubation with increasing dose of 5-FU. The sensitivity to 5-FU and cis-diaminodichloroplatinum (CDDP) was measured by MTT assay. Two dimensional electrophoresis plus mass spectrum(2-DE/MS) was used to identify the differentially expressed protein between Lovo and Lovo/5-FU. The identified protein was then verified by Western blot analysis.</p><p><b>RESULTS</b>The IC50 concentrations of Lovo/5-FU to 5-FU and CDDP were increased by 31 and 3 times, compared with Lovo (both P<0.01). 2DE-MS showed that CAP-G and RhoGDI2 were up-regulated, whereas 6-PGL, DCI, Prdx-6 and Maspin were down-regulated in Lovo/5-FU. Western blot analysis confirmed that the expression levels of RhoGDI2 and CAP-G in Lovo/5-FU were increased by 6.14 and 2.98 fold respectively (both P<0.01), whereas Maspin was decreased to 5.2% of Lovo(P<0.01).</p><p><b>CONCLUSIONS</b>Multi-gene and multi-pathway are involved in the development of multi-drug resistance of colorectal cancer cells. CAP-G, RhoGDI2 and Maspin are potential multi-drug resistant genes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Nuclear Proteins
/
Serpins
/
Colonic Neoplasms
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Cell Line, Tumor
/
Rho Guanine Nucleotide Dissociation Inhibitor beta
/
Genetics
/
Microfilament Proteins
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS