Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro / 生理学报
Acta Physiologica Sinica
;
(6): 464-468, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-290942
ABSTRACT
To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0 10 ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5 10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5 10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Recombinant Proteins
/
Cell Differentiation
/
Cells, Cultured
/
Muscle, Skeletal
/
Ciliary Neurotrophic Factor
/
Cell Biology
/
Myoblasts
/
Cell Proliferation
Limits:
Adult
/
Humans
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2003
Type:
Article
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