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Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro / 生理学报
Acta Physiologica Sinica ; (6): 464-468, 2003.
Article in Chinese | WPRIM | ID: wpr-290942
Responsible library: WPRO
ABSTRACT
To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0 10 ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5 10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5 10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Recombinant Proteins / Humans / Cell Differentiation / Cells, Cultured / Muscle, Skeletal / Adult / Ciliary Neurotrophic Factor / Cell Biology / Myoblasts Language: Chinese Journal: Acta Physiologica Sinica Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Recombinant Proteins / Humans / Cell Differentiation / Cells, Cultured / Muscle, Skeletal / Adult / Ciliary Neurotrophic Factor / Cell Biology / Myoblasts Language: Chinese Journal: Acta Physiologica Sinica Year: 2003 Type: Article