Expression of beta2-adrenergic receptor and its effect on the proliferation of neonatal rat cardiac fibroblasts / 生理学报
Acta Physiologica Sinica
;
(6): 251-254, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-290977
ABSTRACT
The expression of beta-adrenergic receptor subtypes and its effect on neonatal rat cardiac fibroblast proliferation were investigated by radioligand binding assay and [(3)H]-thymidine incorporation analysis, respectively. The results indicated that there was no significant difference in the beta-adrenergic receptor density (B(max)) and affinity (K(D)) between cardiomyocytes and cardiac fibroblasts. The [(125)I]-pindolol competitive inhibition curves (ICI 118551 and CGP 20712A) were significantly better fit in a one-site model in membrane preparation of cardiac fibroblasts. In cultured cardiac fibroblasts, 0.1 micromol/L isoproterenol-induced [(3)H]-thymidine incorporation was completely inhibited by a selective beta (2)-AR antagonist ICI 118551, or a non-selective beta-AR antagonist propranolol, but not by CGP 20712A, a selective beta(1)-AR antagonist. These results suggest that isoproterenol-induced cardiac fibroblast proliferation is mediated by beta(2)-AR, the preponderant beta-AR subtype in cardiac fibroblasts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Cells, Cultured
/
Rats, Wistar
/
Receptors, Adrenergic, beta-2
/
Cell Biology
/
Myocytes, Cardiac
/
Cell Proliferation
/
Fibroblasts
/
Genetics
/
Animals, Newborn
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2003
Type:
Article
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