The studies of hepatitis B virus X gene induces oval cell malignant transformation / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1410-1414, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-291051
ABSTRACT
<p><b>OBJECTIVE</b>To find out the mechanisms of HBx gene inducing oval cell malignant transformation into hepatoma carcinoma cell.</p><p><b>METHODS</b>The changes of morphology, cell cycle, differentiated markers, c-myc and TGF-alpha in pEGFP-HBx oval cells strain, which stably expressed HBx gene, were studied by inversion phase contrast microscope and transmission electron microscopy, flow cytometry, periodic acid-schiff (PAS) staining, soft agar growth assay, real-time PCR, immunocytochemistry. pEGFP-oval cells and LE/6 oval cells were used as control groups.</p><p><b>RESULTS</b>(1) The pEGFP-HBx oval cells showed bigger in size with malformed nucleus as compared with control groups. (2) Flow cytometry showed that, in contrast with the control groups, the proportion of pEGFP-HBx oval cells arrested in G0/G1 phase decreased but in S or G2/M phase rose. Moreover, the population of aneuploid cells increased obviously. (3) PAS staining showed that there were many glycogen granules in the cytoplasm of pEGFP-HBx oval cell. (4) The pEGFP-HBx oval cell formed colonies in the soft agar. (5) Compared with the control groups, the expression of HNF-4 alpha, AFP, c-myc and TGF-alpha rose obviously, whereas the expression of CK-7 and CK-19 decreased. And the expression of cps1 mRNA was not in the extent of detection.</p><p><b>CONCLUSIONS</b>The HBx gene can provoke abnormal differentiation of oval cell and induce oval cell malignant transformation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Transfection
/
Trans-Activators
/
Cell Line
/
Cell Transformation, Neoplastic
/
Hepatocytes
/
Cell Proliferation
/
Genetic Vectors
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2009
Type:
Article
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