Construction of the recombinant adenovirus vector carrying antisense multidrug resistance (MDR1) gene / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 424-427, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-291097
ABSTRACT
The fragment of MDR1 gene obtained from the plasmid pHaMDR1-1 carrying the whole human MDR1 cDNA, was cloned reversely into the shuttle plasmid pAdTrack-CMV. With the resultant plasmid and the backbone plasmid pAdEasy-1, the homologous recombination took place in the Escherichia coli BJ5183 and the recombinant adenoviral plasmid was generated. The adenoviruses were packaged in the 293 cells. The recombinant adenovirus MDR1 vector would introduce the antisense MDR1 gene into the human multidrug resistance hepatocellular cell line effectively, which would provide an experimental basis for studies on the multidrug resistance in human hepatocellular carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plasmids
/
Recombination, Genetic
/
Recombinant Proteins
/
Adenoviridae
/
Oligonucleotides, Antisense
/
Gene Transfer Techniques
/
Carcinoma, Hepatocellular
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Neoplasm
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2004
Type:
Article
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