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Ex vivo studies of ACL and MCL injury of rats under mechanical stretch / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 611-615, 2008.
Article in Chinese | WPRIM | ID: wpr-291180
ABSTRACT
This study sought to detect the pathological changes of anterior cruciate ligament (ACL) and medial collateral ligament (MCL) under injury stretch. Bone-ACL-Bone (B-ACL-B) and B-MCL-B complexes were isolated from 20 male Wister rats, and were immersed in phosphate buffered saline. The complexes were stretched with 10% or 20% strain for 10 min or 30 min. After being stretched, the specimens were fixed in 10% buffered formalin, then mounted in paraffin. Sections were stained with Alcian blue-PAS and HE. The following results were found In the control group, the matrix in ACL contained much more GAGs, as compared with that in MCL. When stretched with 10%, most of the fibroblasts in ACL were elongated like spindles in shape, and some pyknotic nuclei were found increased with stretching time. With 20% strain, ACL showed disruption in parts of collagen fibrils and lysis. But MCL was often torn at its tibia end. The injury can be detected in pathological slices under microscope, even this injury can not be found with naked eye. This injury first starts with the disturbance of the nucleus in the ligament, but following further stretching, it will extend to the rupture of collagen fibrils, and the serious injury of the fibroblasts is especially bad to the repair of the ligament.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Stress, Mechanical / Wounds and Injuries / Anterior Cruciate Ligament / Rats, Wistar / Medial Collateral Ligament, Knee / Anterior Cruciate Ligament Injuries Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Stress, Mechanical / Wounds and Injuries / Anterior Cruciate Ligament / Rats, Wistar / Medial Collateral Ligament, Knee / Anterior Cruciate Ligament Injuries Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2008 Type: Article