The effects of tissue factor/activated factor VII complex on the invasion and metastasis of human ovarian cancer / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 523-527, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-291387
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of tissue factor/activated factor VII (TF/FVIIa) complex in human ovarian cancer invasion and metastasis.</p><p><b>METHODS</b>(1) Constructed an expression vector of TF, pcDNA3-TF and established a human ovarian cell line A2780/TF expressing high level TF by using molecular cloning and gene transfection techniques. (2) By Boyden chamber assay to count the numbers of A2780 and A2780/TF cells that penetrated the matrigel to the back of PVPF membrane after FVIIa stimulation. (3) BALB/c nude mice were used to establish experimental model of metastasis with A2780 or A2780/TF and the lung tissue sections were examined by microscopy for cancer metastasis.</p><p><b>RESULTS</b>(1) Compared with their parental A2780 cells, A2780/TF cells expressed high level of TF mRNA (3.99 +/- 0.15 vs 0.97 +/- 0.23, P < 0.01) and TF antigen on cell surface \[(48.56 +/- 9.53)% vs (2.73 +/- 1.15)%, P < 0.01\]. (2) After stimulation, the A2780/TF cell number on the back of PVPF membrane increased from basal level 157.3 +/- 19.2 to 447.7 +/- 39.4 (P < 0.01), which could decreased to basal level when coincubated with anti-TF antibody. (3) Cancer metastasis was found in 22.2% of nude mice transplanted with A2780 cells, while in 88.9% of those transplanted with A2780/TF cells.</p><p><b>CONCLUSION</b>TF could promote the invasion and metastasis of human ovarian cancer cells through TF/FVIIa pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Pathology
/
Physiology
/
Transplantation, Heterologous
/
Thromboplastin
/
Transfection
/
Factor VIIa
/
Cell Movement
/
Cloning, Molecular
/
Cell Line, Tumor
Type of study:
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article
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