Mutation analysis of SHIP gene in acute leukemia / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 385-388, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-291411
ABSTRACT
<p><b>OBJECTIVE</b>The SH2 domain containing inositol 5'-phosphatase (SHIP) is predominately expressed in hematopoietic cells, and is a crucial negative regulator in the development of hematopoietic cells. This paper is to evaluate the role of the SHIP gene in human leukemogenesis.</p><p><b>METHODS</b>Expression of SHIP gene in bone marrow and/or peripheral blood from 32 patients with acute myeloid leukemia (AML), 9 with acute lymphoblastic leukemia (ALL), as well as human hematopoietic cell lines was analyzed by reverse transcription-polymerase chain reaction (RT-PCR), single strand conformational polymorphism (SSCP) and DNA sequencing.</p><p><b>RESULTS</b>RT-PCR showed that all samples expressed SHIP gene. Mutations of SHIP gene were detected in 7 (22%) of 32 AML patients and one (12%) of 9 ALL patients. Interestingly, two missense mutations that had been observed in a AML patient at diagnosis disappeared after complete remission (CR). In addition, in vitro Akt phosphorylation was prolonged and increased following IL-3 stimulation of this patient's cells.</p><p><b>CONCLUSION</b>Our data demonstrate for the first time the mutation of SHIP gene in acute leukemia and suggest a possible role of the mutation of this gene in the development of acute leukemia. SHIP may serve as a tumor suppressor by negatively regulating the PI3K/Akt signaling pathway in hematopoietic cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphorylation
/
DNA Mutational Analysis
/
Leukemia, Myeloid, Acute
/
Blotting, Western
/
Interleukin-3
/
Phosphoric Monoester Hydrolases
/
Polymorphism, Single-Stranded Conformational
/
HL-60 Cells
/
K562 Cells
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article
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