Hematopoietic stem/progenitor cells and phenotypes of lymphocyte subpopulations in human placenta / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 175-178, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-291422
ABSTRACT
<p><b>OBJECTIVE</b>To study whether human placenta contains hematopoietic stem/progenitor cells (HSPCs), and analyze phenotypes of lymphocyte subpopulations in the placenta.</p><p><b>METHODS</b>Nucleated cells from fresh human placenta were analyzed for phenotypes of HSPCs and lymphocyte subpopulations by flow cytometry (FCM). And CD(34)(+) cells were sorted from human placenta nucleated cells by FCM or MiniMACS.</p><p><b>RESULTS</b>(1) CD(34)(+) cells, CD(34)(+)/CD(38)(+) cells, and CD(34)(+)/CD(38)(-) cells from a human placenta were 8.8, 4.6 and 11.9 times higher than those from umbilical cord blood (UCB), respectively. (2) The yields and purity of CD(34)(+) cells isolated from human placenta by FCM sorting system were (63.05 +/- 10.14)% and (86.39 +/- 11.27)%, respectively. (3) Lymphocytes, T cells (CD(3)(+)/CD(2)(+)), B cells (CD(19)(+)), Th cells (CD(3)(+), CD(4)(+)), and Th/Ts ratio in the placenta tissue were apparently lower than those in the UCB, while the CD(8)(+)/CD(28)(-) T suppressor cells were higher in the placenta than in the UCB.</p><p><b>CONCLUSIONS</b>Human placenta is rich in HSPCs, and has important hematopoietic function in ontogeny. It is probable that human placenta would be graft resource for HSPCs transplantation. CD(8)(+)/CD(28)(-) T suppressor cells might play an important role in feto-maternal immunologic tolerance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Hematopoietic Stem Cells
/
Cells, Cultured
/
Lymphocyte Subsets
/
Lymphocyte Count
/
Cell Biology
/
Allergy and Immunology
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article
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