Effect of ATRA and DNR on the expression and secretion of VEGF in leukemic cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 171-174, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-291423
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cells affected by all-trans retinoic acid (ATRA) and daunorubincin (DNR) respectively.</p><p><b>METHODS</b>Semi-quantitative RT-PCR and ELISA were used to study the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cell lines treated by ATRA and DNR respectively.</p><p><b>RESULTS</b>VEGF was expressed in both NB4 and HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein could be down-regulated by ATRA and DNR respectively in a time and dose dependent manner.</p><p><b>CONCLUSION</b>Besides inducing apoptosis and restraining proliferation of leukemic cells, ATRA and DNR exerted their anti-leukemia effects by reducing angiogenesis via reduction of angiogenic reaction stimulating signals.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Tretinoin
/
Leukemia
/
Daunorubicin
/
Gene Expression Regulation, Leukemic
/
Apoptosis
/
HL-60 Cells
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factors
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article
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