Study of the effect of TF/FVIIa complex on the expression of u-PAR mRNA in human ovarian cancer / 中华血液学杂志
Chinese Journal of Hematology
; (12): 143-146, 2004.
Article
in Zh
| WPRIM
| ID: wpr-291430
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the expression vector of human tissue factor (TF), and investigate the influence of TF/coagulant factor VIIa (FVIIa) complex on the transcriptional expression of urokinase plasminogen activator (u-PA) and u-PA receptor (u-PAR) in human ovarian cancer.</p><p><b>METHODS</b>The human TF cDNA was obtained from placenta by RT-PCR and then inserted into eukaryotic expression vector pcDNA3 to obtain the TF-pcDNA3 combinant. This combinant was transfected into human ovarian cancer cell line A2780 by lipofectamine. Stably-transfected cells A2780/TF were screened. A2780 and A2780/TF cell lines were stimulated by FVIIa respectively, and the transcriptional levels of u-PA and u-PAR were examined by RT-PCR.</p><p><b>RESULTS</b>(1) The constructed product was identified as TF-pcDNA3 combinant by sequencing. (2) TF was highly expressed not only at transcriptional level in the stable-transfected A2780/TF cell (transfected cell 3.91 +/- 0.28, untransfected cell 0.97 +/- 0.23, P < 0.01), but also on the membrane of the cell surface [transfected cell (48.56 +/- 9.53)%, untransfected cell (2.73 +/- 1.15)%, P < 0.01]. (3) The u-PA and u-PAR mRNA levels in A2780 cell line did not change significantly after stimulated by FVIIa; (4) While stimulated by FVIIa, the u-PAR mRNA levels in A2780/TF cells increased significantly in both dose-dependent and time-dependent manner, while the u-PA mRNA levels did not change significantly; (5) In the A2780/TF cell line the enhanced expression of u-PAR mRNA by FVIIa was significantly inhibited by coincubated with anti-TF antibody.</p><p><b>CONCLUSION</b>TF/FVIIa complex could up-regulate the transcription of u-PAR in human ovarian cancer cells so as to enhance tumor invasion and metastasis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Ovarian Neoplasms
/
RNA, Messenger
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Thromboplastin
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Urokinase-Type Plasminogen Activator
/
Factor VIIa
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Gene Expression Regulation, Neoplastic
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Up-Regulation
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Cell Line, Tumor
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Receptors, Urokinase Plasminogen Activator
/
Genetics
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article