Analysis of methylation status of the promoter of mdr1 gene in K562 and K562/DNR cells / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the methylation patterns of mdr1 gene promotor in both K562 cell and K562/DNR cells and the relationship between promotor methylation and P-gp expression.</p><p><b>METHODS</b>mdr1 gene expression was analyzed by flow cytometry and RT-PCR, methylation status of the promotor of mdr1 gene by bisulfite-sequencing (including two GC-box).</p><p><b>RESULTS</b>mdr1 gene was found methylated at GC-box and not expressed in K562 cells, but unmethylated and expressed respectively in K562/DNR cells.</p><p><b>CONCLUSION</b>The methylation patterns of mdr1 gene promotor in K562/DNR and K562 cells were different. mdr1 gene silencing was associated with the promotor methylation.</p>