The initiation of G2/M checkpoint by diallyl disulfide requires the activation of p38 MAP kinase in HL-60 cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 273-276, 2004.
Article
in Zh
| WPRIM
| ID: wpr-291450
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular mechanisms of G(2)/M checkpoint initiated by diallyl disulfide (DADS) in HL-60 cells.</p><p><b>METHODS</b>Cell viability was determined by MTT assay. Cell cycle was assayed by flow cytometry. The expression of phospho-p38, Cdc25B and Cdc2, and p38 mRNA were measured by Western blotting and RT-PCR, respectively.</p><p><b>RESULTS</b>After treatment with DADS at 5 - 160 micro mol/L for 0 - 72 h, the growth of HL-60 cells were suppressed in a concentration-dependent manner and the inhibitory effect of DADS (20 micro mol/L) was similar to that of ATRA (10 nmol/L) (P > 0.05). Incubation of HL-60 cells with DADS (20 micro mol/L) for 12 h could activate G(2)/M checkpoint and increase the expression of phospho-p38 MAPK, followed by the expression of phospho-Cdc25B and phospho-Cdc2 (P < 0.05). SB202190, a specific inhibitor of p38 MAPK, markedly blocked the phosphorylation of p38 MAPK, Cdc25B and Cdc2 (P < 0.05).</p><p><b>CONCLUSION</b>DADS could induce the G(2)/M arrest in HL-60 cells which may be involved in the activation of p38 MAP kinase.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Phosphorylation
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Gene Expression
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Cell Division
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Cell Survival
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Blotting, Western
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G2 Phase
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CDC2 Protein Kinase
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HL-60 Cells
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Reverse Transcriptase Polymerase Chain Reaction
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article