HUP98-HOXA9 transgenic mice are susceptible to N-ethyl-N-nitrosourea stimulation in leukemogenesis / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 257-261, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-291454
ABSTRACT
<p><b>OBJECTIVE</b>In order to investigate the leukemogenic potential of NUP98-HOXA9 fusion gene in vivo.</p><p><b>METHODS</b>Molecular cloning technology was used to construct NUP98-HOXA9 transgenic plasmid and NUP98-HOXA9 transgenic mice were generated. The genotype and phenotype of the NUP98-HOXA9 transgenic mice were analyzed by PCR, RT-PCR and colony-forming assay. The effect of N-ethyl-N-nitrosourea (ENU) stimulation on the transgenic mice was analyzed by peripheral blood count, bone marrow (BM) cells morphology pathological examination.</p><p><b>RESULTS</b>The transgenic expression was detected in 5 independent lines of NUP98-HOXA9 transgenic mice, but no expected phenotypes was found in 2 year follow-up. Upon ENU stimulation, 2 of 10 transgenic mice developed myeloid leukemia, suggesting that NUP98-HOXA9 transgenic mice have increased susceptibility to ENU mutagenesis in leukemogenesis.</p><p><b>CONCLUSION</b>The fusion gene expressed in BM cells of NUP98-HOXA9 transgenic mice. It seems that the expression of the fusion gene is insufficient to trigger leukemogenesis. However, the increased susceptibility to ENU mutagenesis suggests that NUP98-HOXA9 fusion gene might play a potential role in leukemogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Phenotype
/
Plasmids
/
Blood
/
Bone Marrow Cells
/
Mice, Transgenic
/
Transfection
/
Leukemia, Myeloid
/
Gene Expression Regulation, Leukemic
/
Oncogene Proteins, Fusion
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article
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