Nucleotide sequence analysis for a new HLA-B allele HLA-B*13:01:06* / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 790-792, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-291679
ABSTRACT
<p><b>OBJECTIVE</b>To confirm a new allele HLA-B*130106 and analyze its nucleotide sequence.</p><p><b>METHODS</b>Genomic DNA was extracted using a Qiagen DNA extraction kit. Nucleotide sequences of HLA-A, HLA-B, HLA-C and HLA-DRB1 were analyzed by polymerase chain reaction-sequence based typing (PCR-SBT). HLA high-resolution results were assigned, and the nucleotide sequences of HLA-B locus was compared with that of HLA-B*130101.</p><p><b>RESULTS</b>The nucleotide sequence of the new allele shows a strong similarity to that of HLA-B*130101. One nucleotide in exon 2 has changed from G to A at position 219 (codon 49 GCG>GCA), which however did not result in amino acid change.</p><p><b>CONCLUSION</b>The novel allele verified by sequencing has been submitted to GenBank and officially named as HLA-B*130106 by the World Health Organization HLA Nomenclature Committee.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
HLA-B Antigens
/
Exons
/
Amino Acid Sequence
/
Sequence Analysis, DNA
/
Alleles
/
Genetics
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2014
Type:
Article
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