Application of methylation-specific multiplex ligation-dependent probe amplification for the study of DNA methylation in placenta tissues / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the feasibility of using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) for the detection of DNA methylation in placenta tissue.</p><p><b>METHODS</b>For blood cells from 13 non-pregnant women and 9 euploid placenta, the ratios of DNA methylation were evaluated for 4 genes including CGI149, CGI113, HLCS and ACTB with MS-MLPA and bisulfite sequencing, respectively.</p><p><b>RESULTS</b>The methylation ratio of the ACTB gene was 0-0.1 for the blood cells when the digestion control was completely digested. The cutoff value for the methylation ratio of MS-MLPA has been determined as 0.1. For the 9 placenta samples, results of MS-MLPA and bisulfite sequencing were concordant for all of the four genes.</p><p><b>CONCLUSION</b>MS-MLPA is an effective alternative to bisulfite sequencing for the assessment of methylation ratios in placental tissues.</p>