Sequence analysis of a novel allele HLA-C*01:78 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 511-514, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-291739
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic basis for a novel allele HLA-C*0178.</p><p><b>METHODS</b>Genomic DNA was extracted from peripheral blood using a QIAGEN quick DNA extraction kit. The regions encompassing HLA-C from exon 1 to intron 3 and intron 3 to 3'UTR were amplified and cloned using a cloning sequencing kit in order to split the two alleles apart. Selected clones were sequenced to include exons 2 to 4.</p><p><b>RESULTS</b>Sequencing results have indicated the HLA-C alleles of the proband to be a novel C*0304 allele. The sequence has been submitted to GenBank (KF049216). BLAST analysis has confirmed the novel allele to have one nucleotide difference as C*0103 at genomic nt316C>A (codon 82CGC>AGC) in exon 2, which has resulted in replacement of one amino acid (82R>S).</p><p><b>CONCLUSION</b>The novel allele has been officially named as C*0178 by the WHO Nomenclature Committee. The HLA allele type of the proband was therefore A*0207, 2402; B*4001, 4601; C*0178, 0304; DQB1*0502, 0502; DRB1*1602, 1602.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Introns
/
HLA-C Antigens
/
Leukemia
/
Exons
/
Sequence Analysis, DNA
/
Polymorphism, Single Nucleotide
/
Asian People
/
Alleles
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2014
Type:
Article
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