Study of genetic polymorphisms of 12 short tandem repeats on X chromosome in ethnic Han population from Hebei Province / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 388-392, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-291765
ABSTRACT
<p><b>OBJECTIVE</b>To investigate genetic polymorphisms of 12 X chromosome short tandem repeat (X-STR) loci in ethnic Hebei Han population using an Investigator Argus X-12 amplification kit.</p><p><b>METHODS</b>DNA was extracted for 198 unrelated individuals (96 males and 102 females) and amplified with a fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary array electrophoresis.</p><p><b>RESULTS</b>Only DXS10103 and DXS10101 showed significant linkage disequilibrium at the 12 X-STR loci. One hundred and forty-eight alleles, including 22 off-ladder (OL) alleles, were observed at the 12 X-STR loci in the population. The heterozygosity and polymorphic information content (PIC) were 0.5074-0.9143 and 0.4377-0.9079, respectively. The power of discrimination (PD) was 0.5074-0.9143 in males and 0.6876-0.9863 in females. The mean exclusion chance was 0.4377-0.9079 in the trios cases and 0.2984-0.8373 in the duo cases, respectively.</p><p><b>CONCLUSION</b>The Investigator Argus X12 amplification system is highly polymorphic in ethnic Han population from Hebei and is useful for personal identification and paternity testing.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
China
/
Polymerase Chain Reaction
/
Microsatellite Repeats
/
Chromosomes, Human, X
/
Asian People
/
Alleles
/
Ethnology
/
Genetics
/
Genetics, Population
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2014
Type:
Article
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