Effects of tea polyphenols and tea pigments on telomerase activity of HepG2 cells / 中华预防医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>This study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells.</p><p><b>METHODS</b>TRAP-PCR-ELISA was applied to investigate the telomerase activity.</p><p><b>RESULTS</b>Telomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group. Telomerase activities (A(450 approximately 690) values) were 1.56 and 1.46 in 50 mg/L and 100 mg/L tea polyphenols-treated groups, 1.55 and 1.49 in 50 mg/L and 100 mg/L tea pigments-treated groups, respectively. The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group (A(450 approximately 690) = 2.11).</p><p><b>CONCLUSIONS</b>Tea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention.</p>