Experimental studies on cranial defect repair with marrow stromal osteoblast-cancellous bone matrix compound artificial bone / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the feasibility of using marrow stromal osteoblast-cancellous bone matrix compound artificial bone (MCCAB) as tissue-engineered bone, the osteogenesis of MCCAB in the cranial defect was observed in the experiment.</p><p><b>METHODS</b>The in vitro cultivated and induced marrow stromal cells of adult New Zealand rabbits were seeded into the alginate-cancellous bone matrix to form MCCAB. The MCCAB was then implanted into the cranial defect for 4 to 8 weeks. The cancellous bone matrix (CBM) alone or the marrow stromal osteoblasts (MSOs) alone was implanted as the control. The effectiveness of bone formation was assessed by histological and roentgenographic analysis.</p><p><b>RESULTS</b>The osteogenesis of MCCAB was better than CBM or MSOs and superior to the blank group.</p><p><b>CONCLUSION</b>MCCAB can effectively repair cranial defect. It could be used clinically to restore large bone defects.</p>