The experimental study on antisense TGF-beta 1 in inhibiting scar formation in III degree burn wound / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of antisense TGF-beta 1 in inhibiting scar formation during wound healing.</p><p><b>METHODS</b>SD rats were divided into three groups after skin burn: group one was treated with antisense TGF-beta 1 oligonucleotide; group two was treated with antisense TGF-beta 1 recombinant plasmid and the control group. In different time, RT-PCR and immunohistochemistry were used to verify the expression of TGB beta 1 mRNA and protein. Type I Collagen mRNA expression was determined by hybridization in situ. Inflammatory reaction and collagen distribution were observed pathologically.</p><p><b>RESULTS</b>In the groups received antisense ODN and recombinant plasmid, the expression of TGF-beta 1 mRNA and protein reduced during 14 days after burn. In the control group, type I collagen mRNA began to express at the second week and reached a peak at the fourth week, while the antisense groups kept low expression. The antisense group also showed mild inflammatory reaction and less synthesis of collagen.</p><p><b>CONCLUSION</b>Antisense TGF-beta 1 could prevent the scar formation during wound healing.</p>