Influence of Japanese enciphalitis virus capsid protein on the self-replicate ability of JEV replicon vectors / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1088-1094, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-292167
ABSTRACT
To optimize a self-replicate Japanese enciphalitis virus (JEV) replicon, and to make it as an efficient vector to express the heterologous protein, we constructed three JEV replicons by PCR-based shortening the length of capsid genes. The vectors remained full or part of C gene, based on the JEV replicon pCTCJEV. Lac Z was selected as the reporter gene to verify the self-replicate ability of these DNA-based replicons. While transfected into the cell lines CME-4, which continuously expressing the JEV structure proteins C-prM-E, the JEV replicons pCMW-2M-1LACZ, pCMW-2M-3LACZ, which remained the first 23aa and 68aa of C protein, can express the reporter protein as the same level as pCMW-2M-LACZ with the full-length C protein. These results illustrated that the JEV replicon vector with 69-nt of the C gene can retain the self-replicate ability, and provide valuable tools to construct a possible vector for a long-lasting JEV RNA virus expression system.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Replicon
/
Virus Replication
/
Transfection
/
Cell Line
/
Capsid Proteins
/
Encephalitis Virus, Japanese
/
Genetic Vectors
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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