Effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm.</p><p><b>METHODS</b>Different proportions of cryoprotectant to seminal plasma (11 and 13) were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared.</p><p><b>RESULTS</b>The forward movement and total motility rates were (58.60 +/- 5.57)% and (66.17 +/- 5.24)% before cryopreservation. The 11 proportion achieved post-thaw forward movement and total motility rates of (40.53 +/- 8.97)% and (51.23 +/- 9. 30)%, while the 13 (44.7 +/- 8.67)% and (51.50 +/- 7.40)%, respectively. Significantly decreased sperm motility was observed after cryopreservation (P < 0.05). Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions.</p><p><b>CONCLUSION</b>Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma (13) can improve the forward movement of post-thaw sperm as compared with the lower one (11).</p>