Effects of phytoestrogens on testosterone production of rat Leydig cells / 中华男科学杂志
National Journal of Andrology
;
(12): 207-211, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-292398
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of phytoestrogens (daidzein and genistein) on the testosterone production of rat Leydig cells and the possible mechanisms.</p><p><b>METHODS</b>Primary Leydig cells were obtained from 3-month old male SD rats using discontinuous Percoll density gradient centrifugation. The effects of phytoestrogens at various concentrations were evaluated by ELISA, with hCG as the positive control. The mRNA expression of P450 side-chain cleavage enzyme (P450scc) was analyzed by semi-quantitative RT-PCR.</p><p><b>RESULTS</b>Genistein at 0.1 micromol/L obviously promoted the secretion of testosterone and upregulated the mRNA level of P450scc. At a higher concentration of 5 micromol/L, however, both daidzein and genistein significantly inhibited the testosterone production of Leydig cells (P > 0.05).</p><p><b>CONCLUSION</b>Genistein can promote the testosterone production of Leydig cells at a low concentration (0.1 micromol/L), but both daidzein and genistein can inhibit it at a higher concentration ( >5 micromol/L).</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Testosterone
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Bodily Secretions
/
Genistein
/
Phytoestrogens
/
Isoflavones
/
Leydig Cells
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2009
Type:
Article
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