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1,5-dicaffeoylquinic acid protects primary neurons from amyloid β 1-42-induced apoptosis via PI3K/Akt signaling pathway / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 2628-2635, 2011.
Article in English | WPRIM | ID: wpr-292832
ABSTRACT
<p><b>BACKGROUND</b>Recently, 1,5-dicaffeoylquinic acid (1,5-DQA), a caffeoylquinic acid derivative isolated from Aster scaber, was found to have neuroprotective effects. However, the protective mechanisms of 1,5-DQA have not yet been clearly identified. The purpose of this study was to explore the protective mechanisms of 1,5-DQA on neuronal culture.</p><p><b>METHODS</b>We investigated the neuroprotective effects of 1,5-DQA against amyloid β(1-42) (Aβ(42))-induced neurotoxicity in primary neuronal culture. To evaluate the neuroprotective effects of 1,5-DQA, primary cultured cortical neurons from neonate rats were pretreated with 1,5-DQA for 2 hours and then treated with 40 µmol/L Aβ(42) for 6 hours. Cell counting kit-8, Hoechst staining and Western blotting were used for detecting the protective mechanism. Comparisons between two groups were evaluated by independent t test, and multiple comparisons were analyzed by one-way analysis of variance (ANOVA).</p><p><b>RESULTS</b>1,5-DQA treated neurons showed increased neuronal cell viability against Aβ(42) toxicity in a concentration-dependent manner, both phosphoinositide 3-kinase (PI3K)/Akt and extracellular regulated protein kinase 1/2 (Erk1/2) were activated by 1,5-DQA with stimulating their upstream tyrosine kinase A (Trk A). However, the neuroprotective effects of 1,5-DQA were blocked by LY294002, a PI3K inhibitor, but not by PD98059, an inhibitor of mitogen-activated protein kinase kinase. Furthermore, 1,5-DQA's anti-apoptotic potential was related to the enhanced inactivating phosphorylation of glycogen synthase kinase 3β (GSK3β) and the modulation of expression of apoptosis-related protein Bcl-2/Bax.</p><p><b>CONCLUSION</b>These results suggest that 1,5-DQA prevents Aβ(42)-induced neurotoxicity through the activation of PI3K/Akt followed by the stimulation of Trk A, then the inhibition of GSK3β as well as the modulation of Bcl-2/Bax.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Signal Transduction / Cell Survival / Cells, Cultured / Blotting, Western / Amyloid beta-Peptides / Cinnamates / Rats, Sprague-Dawley / Apoptosis / Phosphatidylinositol 3-Kinases Limits: Animals Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Signal Transduction / Cell Survival / Cells, Cultured / Blotting, Western / Amyloid beta-Peptides / Cinnamates / Rats, Sprague-Dawley / Apoptosis / Phosphatidylinositol 3-Kinases Limits: Animals Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article