Your browser doesn't support javascript.
loading
Effects of sex hormones on genioglossal muscle activities, estrogen and androgen receptor expression in adult rat / 中华口腔医学杂志
Chinese Journal of Stomatology ; (12): 85-89, 2007.
Article in Chinese | WPRIM | ID: wpr-292958
ABSTRACT
<p><b>OBJECTIVE</b>To explore gene expression of estrogen receptor (ERalpha, ERbeta) and androgen receptor (AR) in genioglossal muscle (GG) of adult male rats, and to investigate the effects of sex hormones on GG activities, ERalpha, ERbeta and AR expression.</p><p><b>METHODS</b>GG samples were collected from 10 healthy adult male rats. Total RNA were extracted and subjected to fluorescent quantitative reverse transcription-polymerase chain reaction (FQ RT-PCR) for quantitative measurement of ERalpha, ERbeta and AR mRNAs. The other 24 male rats were randomly divided into 3 groups control group, estrogen group (intramuscular injection of estrogen 0.1 mg/kg, twice a week) and androgen group (intramuscular injection of androgen 2.5 mg/kg, twice a week). The electromyographic activities (EMG) and contract tension of GG were investigated after 4-week treatment. The expression of ERalpha, ERbeta and AR was assessed by Western blot.</p><p><b>RESULTS</b>The mRNA expression ratios of AR/GAPDH, ERalpha/GAPDH, ERbeta/GAPDH and ERalpha/ERbeta were (295.80 +/- 127.20), (2042.00 +/- 921.57), (65.96 +/- 29.57) and (36.83 +/- 19.66), respectively. The mRNA level of ERalpha was significantly higher than that of ERbeta (P < 0.01). Compared with the control group, the EMG of GG was intensified in the estrogen group (P < 0.01). GG contractility did not change significantly (P > 0.05), and ERalpha expression in GG was up-regulated by estrogen (P < 0.05); while in the androgen group, the EMG of GG was weakened (P < 0.05). P(t) and P(0) were slightly increased (P > 0.05) and the decline rate of P(0) was markedly quickened (P < 0.05). AR and ERbeta expressions were down-regulated by androgen (P < 0.05).</p><p><b>CONCLUSIONS</b>Both AR and ER were expressed in GG of adult male rats, and ERalpha was expressed more abundantly than ERbeta. Estrogen could greatly improve activities of GG and stimulate the expression of ERalpha. Whereas, androgen could restrain activities of GG, impair its fatigue resistance capacity and inhibit the expression of AR and ERbeta.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Pharyngeal Muscles / Physiology / Tongue / RNA, Messenger / Random Allocation / Receptors, Androgen / Receptors, Estrogen / Gene Expression Regulation / Rats, Sprague-Dawley Limits: Animals Language: Chinese Journal: Chinese Journal of Stomatology Year: 2007 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Pharyngeal Muscles / Physiology / Tongue / RNA, Messenger / Random Allocation / Receptors, Androgen / Receptors, Estrogen / Gene Expression Regulation / Rats, Sprague-Dawley Limits: Animals Language: Chinese Journal: Chinese Journal of Stomatology Year: 2007 Type: Article