Experimental study on dog's bone marrow stem cells transfected by pIRES2-EGFP-IGF-1 gene / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 739-742, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-292975
ABSTRACT
<p><b>OBJECTIVE</b>To establish the bone marrow stem cells (MSC) model which could highly express the insulin-like growth factor 1 (IGF-1) transfected by dog's IGF-1 gene.</p><p><b>METHODS</b>pIRES2-EGFP-IGF-1 was transfected into MSC by lipofectamine. Positive clones were selected with G418. The expression of IGF-1 protein in the MSC was determined by immunohistochemistry and Western blot analysis. The IGF-1 in the supernatant of the transfected MSC was detected by sandwich-in ELISA. The periodontal ligament cells (PDLC) were cultured in the supernatant of the transfected MSC. The changes of PDLC' proliferation were observed by MTT.</p><p><b>RESULTS</b>IGF-1-transfected MSC could apparently express IGF-1. The IGF-1 protein in the supernatant of the transfected MSC was confirmed by sandwich-in ELISA. IGF-1 could promote the PDLC' proliferation.</p><p><b>CONCLUSIONS</b>The MSC transfected by dog's IGF-1 gene can highly express IGF-1, which may lay the foundation for further study on periodontal regeneration.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Insulin-Like Growth Factor I
/
Bone Marrow Cells
/
Transfection
/
Cells, Cultured
/
Cell Biology
/
Mesenchymal Stem Cells
/
Genetic Vectors
/
Genetics
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2006
Type:
Article
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